小麦成株期白粉病抗性位点的QTL定位

惠建,白海波,马斯霜,陈晓军,吕学莲,李树华

植物遗传资源学报. 2021, 22(5): 1348-1354

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植物遗传资源学报 ›› 2021, Vol. 22 ›› Issue (5) : 1348-1354. DOI: 10.13430/j.cnki.jpgr. 20210531001
论文

小麦成株期白粉病抗性位点的QTL定位

  • 惠建, 白海波, 马斯霜, 陈晓军, 吕学莲, 李树华
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QTL Mapping for Wheat Powdery Mildew Resistance at the Adult Stage

  • HUI Jian, BAI Hai-bo, MA Si-shuang, CHEN Xiao-jun, LYU Xue-lian, LI Shu-hua
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摘要

基于高感白粉病小麦品种宁春4号和高抗白粉病品种宁春27号构建的重组自交系群体(RIL),以及简化基因组测序方法获得的遗传连锁图谱,通过QTL定位分析了小麦成株期白粉病抗性基因。结果表明:4对主基因控制的加性-上位性遗传模型适合于RIL群体的成株期白粉病抗性;2个稳定的抗病QTL QPm.naafs-4D和QPm.naafs-7D的分别来自宁春27号和宁春4号,其中在不同环境下QPm.naafs-4D的LOD为5.9 ~ 15.4、加性效应为-1.1 ~ -1.2、表型变异解释率为18.6% ~ 34.5%,该位点与侧翼标记的遗传距离为4 cM。通过序列比对确认QPm.naafs-4D的抗性基因即为持久多抗基因Pm46/Lr67/Yr46/Sr55,它可直接应用于小麦抗白粉病分子辅助育种。

Abstract

This study reported a quantitative trait locus (QTL) mapping for identifying the adult-stage powdery mildew resistance genes in a recombinant inbred line (RIL) population of common wheat (Triticum aestivum L.). This population was derived from the highly susceptible variety Ningchun 4 crossing with the highly resistant variety Ningchun 27, and genotyped using genotyping-by-sequencing (GBS) approach. The results showed that the additive-epistatic genetic model using four pairs of major genes was suitable for identifying the powdery mildew resistance loci. Two resistance-conferring loci QPm.naafs-4D and QPm.naafs-7D have been identified from Ningchun 27 and Ningchun 4, respectively. Out of them, QPm.naafs-4D represented a LOD value of 5.9 to 15.4 at multiple environmental conditions (additive effects: -1.1 to -1.2), and contributed to a phenotypic variation of 18.6% to 34.5%. This locus was delimited to a genetic distance of 4 cM harboring the durable poly-resistance gene Pm46/Lr67/Yr46/Sr55. Sequence analysis confirmed that QPm.naafs-4D in Ningchun 27 was identical to Pm46/Lr67/Yr46/Sr55. The resistance-conferring donor line is valuable in marker-assisted breeding for powdery mildew resistance in wheat.

关键词

小麦 / 白粉病抗性 / 成株期 / 遗传模型 / QTL定位

Key words

Wheat;Powdery mildew resistance;Adult stage;Genetic model;QTL mapping

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惠建,白海波,马斯霜,陈晓军,吕学莲,李树华. 小麦成株期白粉病抗性位点的QTL定位. 植物遗传资源学报. 2021, 22(5): 1348-1354 https://doi.org/10.13430/j.cnki.jpgr. 20210531001
HUI Jian,BAI Hai-bo,MA Si-shuang,CHEN Xiao-jun,LYU Xue-lian and LI Shu-hua. QTL Mapping for Wheat Powdery Mildew Resistance at the Adult Stage. Journal of Plant Genetic Resources. 2021, 22(5): 1348-1354 https://doi.org/10.13430/j.cnki.jpgr. 20210531001

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